Rooperol and its derivates

ABSTRACT

The invention relates to a new anti-cancer chemical compound which has been called rooperol and certain derivatives thereof of the general formula ##STR1## in which A is chosen from the group including a phenyl group, a substituted phenyl group and --CH 2  --O--R 5  where R 5  =H, an alkyl (C 1  -C 5 ), aralkyl or acyl substituent; R 1  and R 2  are chosen from substituents including H for both or singly if one of them is --OH, --NH 2 , --SH, ##STR2## or taken together R 1  and R 2  are =0; R 3  is H or ##STR3## where R 6  is an alkyl group (C 1  -C 7 ); either of R 4  or B are chosen from substituents including H, ##STR4## a phenyl group, substituted phenyl group or a furyl group.

This is a continuation-in-part of co-pending application Ser. No. 624,717 filed on June 23, 1984, now abandoned.

FIELD OF THE INVENTION

This invention relates to the chemical compound rooperol and to related compounds and their derivatives.

Rooperol has the formula: ##STR5##

BACKGROUND OF THE INVENTION

Hypoxoside, (see below for structure), has been described in EPO Patent Application No. 83103765.0 and was obtained by the extraction of plants of the Hypoxidaceae family. In this patent application the applicant reported that hypoxoside has anticancer activity.

Extracts of plants of the Hypoxis genus (Hypoxidaceae) have been found to be active in tests against Mouse P388 lymphocytic leukemia cell cultures (A. Barclay and R. C. Perdue: Distribution of anticancer activity in higher plants. Cancer Treatment Reports, 1976, 60, 1081-1113 (I)). In addition, extracts of Hypoxis obtusa have been reported to have been used by certain African people for combating urinary diseases and the isolation of the acetylenic compound hypoxoside from this plant has been reported (G. B. Marini Bettolo, M. Patamia, M. Nicoletti, C. Galeffi and L. Messana: Tetrahedron, 1982, 38, 1683-1687 (II).

Acetylenic compounds occur widely in nature. Because of their antifungal, bacteriostatic and herbicidal properities as well as their potential application in medicine as hypnotic, sedative, anticonvulsant, analgesic anti-inflammatory and hypotensive agents considerable interest has been shown in the syntheses of these compounds. Numerous reviews cover the field of both natural and synthetic acetylenic compounds (the most comprehensive of these reviews are by K. E. Schulte and G. Rucker: Synthetische and naturliche Acetylen-Verbindungen als Arzneistoffe.--Progr. Drug Res 1970, 14, 387-563 (III) and by O. G. Yashina and L. I. Vereshchagin: Natural and Synthetic Acetylenic Antimycotics.--Russian Chem. Revs. (Transl.) 1978, 47, 307-317 (IV). Some acetylenic substances, although not structurally related to the compounds of the invention, have been found to be cytostatic and some have been used in the treatment of cancer (ref III). In addition some natural and synthetic acetylenoids have the pent-4-en-1-yne central unit.

Although these particular compounds possess some of the above mentioned biological properties none of them has been reported to be cytostatic nor have they been described as potential or actual anticancer agents (refs III and IV). Compounds of this invention that have been previously synthesized are compound 3 (J. Klein and S. Brenner: J.S.C.S., 1969, 91, 3094-3096 (V) and by V. Grignard: Bull. Soc. Chim. Fr., 1928, 43, 141-142, Compound 2 (ref. V) and Compounds 16 and 25 (ref. IV), but the quoted patent (DE No. 2025755-GB No. 1284475-U.S. Pat. No. 3,794,689. Chem Abstr., 1971, 74, 53260) describes different though closely related, substances: (HC.tbd.C)₂ C(OH).Ar.

It is the object of the present invention to prepare rooperol and describe its properties together with the preparation and properties of othe related compounds and derivatives; and secondly to describe the use of these compounds as anticancer agents.

THE INVENTION

According to the invention there is provided a group of compounds having the general formula: ##STR6## in which A is chosen from the group including a phenyl group, a substituted phenyl group and --CH₂ --O--R⁵ where R⁵ =H, an alkyl (C₁ -C₅), aralkyl or acyl substituent; R¹ and R² are chosen from substituents including H for both or singly if one of them is --OH, --NH₂, --SH, ##STR7## or taken together R¹ and R² are =0; R³ is H or ##STR8## where R⁶ is an alkyl group (C₁ -C₇); either of R⁴ or B are chosen from substituents including H, ##STR9## a phenyl group, substituted phenyl group or a furyl group.

In one aspect of the invention A and/or B may be the group: H, alkyl (straight chain, branched or cyclic) or aromatic including heterocyclic systems. All these terminal groups may have functional group substituents in various positions.

The compound, Hypoxoside, was obtained from Hypoxis rooperi, H. nitida, H. obtusa, H. rigidula, H. latifolia and other Hypoxidaceae species. Hypoxoside may be named as a derivative of pentane as follows: ##STR10## (E)-1,5-bis(3',4'-dihydroxyphenyl)pent-4-en-1-yne 4',4'-di-B-D-glucopyranoside.

A number of examples of compounds according to the invention are listed in the following tables (1(a) and 1(b)).

       PRECURSORS AS ILLUSTRATED   PRODUCT IN SCHEME PRODUCT mp/bp No. X Z Y      FORMULA *nmr              1       ##STR11##       C.sub.17 H.sub.14 O.sub.4 148°       2      ##STR12##       ##STR13##       ##STR14##       C.sub.11 H.sub.10 103°/20 Torr       3      ##STR15##       ##STR16##       ##STR17##       C.sub.17 H.sub.14 176/2 Torr       4      ##STR18##       ##STR19##       ##STR20##       C.sub.18 H.sub.14 O.sub.2 49-50°       5      ##STR21##       ##STR22##       ##STR23##       C.sub.19 H.sub.18 O.sub.2 45°       6      ##STR24##       ##STR25##       ##STR26##       C.sub.18 H.sub.16 O oil       7      ##STR27##       ##STR28##       ##STR29##       C.sub.19 H.sub.14 O.sub.4 59-60°       8      ##STR30##       ##STR31##       ##STR32##       C.sub.21 H.sub.22 O.sub.4 *nmr       9      ##STR33##       ##STR34##       ##STR35##       C.sub.19 H.sub.18 O.sub.2 *nmr       10      ##STR36##       ##STR37##       ##STR38##       C.sub.17 H.sub.13 Br 65-70°       11      ##STR39##       ##STR40##       ##STR41##       C.sub.11 H.sub.11 OCl *nmr       12      ##STR42##       ##STR43##       ##STR44##       C.sub.17 H.sub.20 O.sub.2 *nmr       13      ##STR45##       ##STR46##       ##STR47##       C.sub.11 H.sub.16 O.sub.2 *nmr       14      ##STR48##       ##STR49##       ##STR50##       C.sub.18 H.sub.14 O.sub.2 49-50°       15      ##STR51##       ##STR52##       ##STR53##       C.sub. 17 H.sub.16 O.sub.4 *nmr       16      ##STR54##       ##STR55##       ##STR56##       C.sub.17 H.sub.14 O 69-71°       17      ##STR57##       ##STR58##       ##STR59##       C.sub.15 H.sub.12 O.sub.2 *nmr       18      ##STR60##       ##STR61##       ##STR62##       C.sub.19 H.sub.18 O.sub.3 *nmr       19      ##STR63##       ##STR64##       ##STR65##       C.sub.17 H.sub.13 OCl *nmr       20      ##STR66##       ##STR67##       ##STR68##       C.sub.11 H.sub.10 O 98°/1,5 Torr       21      ##STR69##       ##STR70##       ##STR71##       C.sub.12 H.sub.18 O.sub.3 *nmr       22      ##STR72##       ##STR73##       ##STR74##       C.sub.21 H.sub.22 O.sub.5       23      ##STR75##       ##STR76##       ##STR77##       C.sub.20 H.sub.18      *nmr data per table 1 (c)

DERIVATIVES OF PRODUCT NOS. 1 TO 23

    __________________________________________________________________________     PRODUCT                                                mp/bp                   No.    PRODUCT                                   FORMULA                                                                              *nmr                    __________________________________________________________________________     24                                                                                     ##STR78##                                C.sub.11 H.sub.10                                                                    112°/25                                                                 Torr                    25                                                                                     ##STR79##                                C.sub.17 H.sub.12                                                                    69-71°           26                                                                                     ##STR80##                                C.sub.11 H.sub.8                                                                     105°/2                                                                  Torr                    27                                                                                     ##STR81##                                C.sub.25 H.sub.22                                                              O.sub.8                                                                              amorph                  28                                                                                     ##STR82##                                C.sub.49 H.sub.54                                                              O.sub.24                                                                             134°             29                                                                                     ##STR83##                                C.sub.7 H.sub.10                                                                     *nmrb.2                 30                                                                                     ##STR84##                                C.sub.6 H.sub.8 O                                                                    60°/0.75                                                                Torr                    31                                                                                     ##STR85##                                C.sub.12 H.sub.12                                                                    127°/0.75                                                               Torr                    __________________________________________________________________________      *nmr data per table 1 (c)                                                

NMR DATA Table 1(c) Product 8

δ_(H) (CDCl₃ ; 80 MHz; TMS) 3.32 (2H, dd, J₁ =5.4 Hz J₂ =<0.1 Hz, CH₂ --CH═CH), 3.85 (6H, s, 2 OCH₃) 3.88 (6H, s, 2 OCH₃), 5.99 and 6.19 (1H, dt, J₁ =15 Hz J₂ =5,4 Hz, CH₂ CH═CH), 6.62 (1H, d, J=15 Hz, CH═CH--ArH), 6.94 (6H, m, ArH).

Product 9

δ_(H) (CDCl₃ ; 60; mHz; TMS), 3.33 (2H, dd, J₁ =5,0 Hz J₂ =<0.1 Hz CH₂), 3.85 (6H, s, OCH₃), 6.06 and 6.32 (1H, dt, J₁ =15.8 Hz J₂ =5,0 Hz --CH--CH₂ --), 6.55-7.43 (9H, m, CH--ArH)

Product 11

δ_(H) (CDCl₃ ; 60 MHz; TMS) 2.7 (2H, d, J=6.1 Hz, C.tbd.C--CH₂), 2.85 (1H, s, OH), 3.5-3.75 (2H, m, CH₂ Cl), 3.85-4.2 (1H, m, CH--CH₂ Cl), 7.05-7.5 (5H, m, ArH).

Product 12

δ_(H) (CCl₄ ; 60 MHz; TMS) 1.23-1.80 (6H, m, THP,β,β',γ, CH₂) 3.08 (2H, dd, J₁ =4 Hz J₂ =1.5 Hz C.tbd.C--CH₂ --), 3.40-3.80 (2H, m, THP,α,CH₂), 4.27 (2H, t, J=2 Hz O--CH₂), 4.73-4.90 (1H, m, THP,α,CH), 5.92 and 6.21 (1H, dt, J₁ =15.8 Hz J₂ =5,4 Hz, CH₂ --CH.tbd.C), 6.61 (1H, d, J=15.8 Hz CH--ArH), 7.08-7.37 (5H, m, ArH).

Product 13

δ_(H) (CCl₄ ; 60 MHz; TMS) 1.55 (6H, m, THP, β,β',γ,CH₂) 2.9 (2H, m, C.tbd.C--CH₂), 3.3-3.9 (2H, m, THP,α,CH₂), 4.1 (2H, m, OCH₂ C.tbd.C), 4.7 (1H, s, THP,αCH), 5.05 and 5.3 (2H, d, J=9.1 Hz, CH═CH₂), 5.45-6.0 (1H, m, CH═CH₂).

Product 15

δ_(H) (CDCl₃ ; 60 MHz; TMS) 2.65 (1H, s, OH), 3.8 (6H, s, 2 OCH₃), 5.2 (1H, d, J=5.7 Hz, C.tbd.C--CH), 6.05-7.3 ##STR86##

Product 17

δ_(H) (CDCl₃ ; 60 MHz; TMS) 2.65 (1H, s, OH), 5.2 (1H, d, J=5.9 Hz, CH--OH), 6.0-6.7 ##STR87## 7.0-7.6 (5H, m, ArH).

Product 18

δ_(H) (CDCl₃ ; 60 MHz; TMS) 3.8 (6H, s, 2OCH₃), 5.2 (1H, d, C.tbd.C--CH), 6.4 and 6.7 (2H, dd, J₁ =6.9 Hz J₂ =<0.1 Hz, CH═CH), 6.9 (1H, s, OH), 7.0-7.5 (8H, m, ArH).

Product 19

δ_(H) (CDCl₃ ; 60 MHz; TMS) 2.6 (1H, s, OH), 5.7 (1H, d, J=7.9 Hz, C.tbd.C--CH), 6.3 (1H, d, J=8.0 Hz, CH═C), 7.1-7.7 (10H, m, ArH).

Product 21

δ_(H) (CCl₄ ; 60 MHz; TMS) 1.6 (6H, m, THP,β,β',γ,CH₂), 1.7 (3H, d, J=6.2, Hz CH₃), 3.3-4.0 (3H, m, THP,α,CH₂ and OH), 4.2 (2H, m, --CH₂ --), 4.75 (2H, m, C.tbd.C--CH and THP,α,CH) 5.3-6.1 (2H, m, CH═CH).

Product 23

δ_(H) (CDCl₃ ; 60; MHz; TMS), 1.36 (3H, t, J=7.5 Hz, CH₃), 3.63 (2H, s, CH₂ --C.tbd.C), 4.33 (2H, d, J=7.5 Hz CH₂ --CH₃) 7.17-7.67 (10H, m, ArH) 7.83 (1H, s, C═CH)

Product 29

δ_(H) (CDCl₃ ; 60 MH; TMS) 1.3 (3H, d, J=6.1 Hz, CH₃), 3.1 (2H, s, 2 OH), 4.15-4.5 (3H, m, CH₂ OH and C.tbd.C--CH), 5.5 and 5.75 (1H, m, CH₃ CH═CH), 6.0 and 6.3 (1H, dd, J₁ =5.0 Hz J₂ =<0.1 Hz, CH═CH--CH₃).

SCHEME

The compounds of the invention may be synthesised by the following scheme, in which A, B, R¹, R², R³ and R⁴ have the meaning described above ##STR88##

GENERAL METHODS

1. Bromoethane (1 mol equiv) is added dropwise to a stirred mixture of magnesium metal (1.05 mol equiv) and THF under a N₂ atmosphere. Once the exothermic reaction has subsided, the reaction mixture is refluxed for 10 minutes, cooled to 20° C. and the alkyne X (as in Scheme) (1.05 mol equiv) added dropwise. Ethane is evolved. The mixture is then refluxed for 45 min., and cooled to 20° C. This provides anion Q in the reaction mixture as shown in the scheme.

2. Products involving alkyl halide (Z) precursors:

Cuprous chloride (CuCl) is added to the Q containing mixture which is then stirred for 15 min. before the alkyl halide (Z) (1.05 mol equiv) is added dropwise. The green suspension is refluxed for 45 min. before aqueous ammonium chloride and KCN is added followed by extraction.

3. Other products involving precursors Z

The mixture which contains anion Q is cooled to 0° C. and the compound Z (as in Scheme) is added dropwise maintaining the temperature between 0° C. and 5° C. during this procedure. Following addition of all the compound Z the mixture is stirred for 21/2 hours at 25° C. and then poured into a saturated aqueous solution of NH₄ Cl followed by extraction.

PREPARATION OF ROOPEROL PRODUCT (1)

I. Hydrolysis of Hypoxoside (100 mg), in distilled water (6 ml, pH 6.3) with β-glucosidase (100 mg in 4 ml H₂ O), at 37° C. provided to be satisfactory. The 3',3',4',4',-tetrahydroxyrooperol (1) had mp 148° (lit. mp 154°-156°, ref. II).

II. Tetramethoxy product (8) (0,0075 mol), dry quinoline (0.25 mol) and TMSI (0.046 mol of ˜92.5% solution) were added together under a nitrogen atmosphere and heated at 180° C. for 70 min. After cooling and addition of 5% HCl the dark mixture was extracted with ether, the combined ether extracts were washed with 5% HCl (˜550 ml). The washed ether extract was dried (Na₂ SO₄), concentrated in vacuo, then heated at 40°-50° C. in methanol plus a few drops of water, until t.l.c. (benzene/acetone) (7:3) showed the high R_(F) tetra (trimethyl silyl) ether of Product 1 to have disappeared with only Product 1 showing (c.f. Product 1 from plant material). The solvent was removed in vacuo at room temperature followed by purification of the residue on a medium pressure liquid chromatography column, eluting with benzene/acetone (7:3).

IN VITRO EFFECTS OF SOME PENT-4-EN-1-YNES AND DERIVATIVES ON CELL CULTURES

The cell cultures were grown under standard conditions at 37° C. in Eagle's Minimal Essential Medium (M.E.M., Gibco), with glutamine and non-essential amino acids, supplemented with 10% foetal calf serum (State Vaccine Institute, Cape Town). Cells for subcultures were obtained from 90-100% confluent cell cultures by trypsinisation with Ca⁺⁺ Mg⁺⁺ --free EDTA-Dulbecco Buffer, containing 0.25% trypsin. Trypsin was inactivated by placing the harvested cell suspension into M.E.M. containing 10% foetal calf serum. The cell suspensions were then suitably diluted to the final volume, depending on the number of cells obtained and the growth profile of the cell type. The diluted cell suspensions were then dispensed at the rate of 1.0 ml into prepared cell culture flasks containing 8.9 ml culture media. These cultures were incubated for 24 hours and the media changed before adding the test compounds.

The test compounds were dissolved in dimethylsulphoxide (Merck) and added at the rate of 0.1 ml to the media in a dilution to give the desired end concentration.

In each series of experiments, control cultures were treated with equivalent amounts of solvent without the test compound. The cultures were examined daily usually for a period of four days and cell growth and other cytopathic effects were records. Abnormal cell patterns and sizes were also determined during counting.

These included:

    ______________________________________                                         (i)   M (52) B       Mouse Sarcoma                                             (ii)  Mel B16 BL06   Mouse Melanoma                                            (iii) HOC            Human Oesophagal Carcinoma                                (iv)  HeLa           Human Cervical Cancer                                           ATCC No. CCL2                                                            (v)   P27            Human Mesothelioma Cells                                  (vi)  "Loots" cells  Human Derived Adenocarcinoma                              (vii) Chang          Chang Conjunctival cells                                        ATTC No. CCL20.2                                                         ______________________________________                                    

The results are presented in tables 2 to 8.

ACUTE TOXICITY EVALUATION OF SOME PENT-4-EN-1-YNES AND DERIVATIVES

Some compounds were subjected to preliminary acute toxicity tests in mice and rats. Solutions or suspensions of the selected compounds were prepared in vegetable oil. The volumes administered were kept constant at 10.0 ml per kg. In each case equal numbers of male and female animals were treated with a single dose. All animals were observed over 7 days for signs of toxic and/or pharmacodynamic effects. Necropsies were performed on all surviving animals. Results are presented in table 9.

                  TABLE 2                                                          ______________________________________                                         INHIBITION OF CELL GROWTH: MOUSE SARCOMA                                       M(52)B                                                                                  MINIMUM INHIBITORY CONCENTRA-                                                  TIONS (RANGE) WHICH PRODUCE AP-                                       PRODUCT  PROXIMATELY 50% CELL GROWTH RE-                                       No.      DUCTION μg/ml MEDIA                                                ______________________________________                                         1        12.5-25.0                                                             3        50.0-75.0                                                             7        60.0-80.0                                                             8        60.0-80.0                                                             16       20.0-40.0                                                             23        75.0-100.0                                                           ______________________________________                                    

                  TABLE 3                                                          ______________________________________                                         INHIBITION OF CELL GROWTH: MOUSE MELANOMA                                      B16-BL6                                                                                 MINIMUM INHIBITORY CONCENTRA-                                                  TIONS (RANGE) WHICH PRODUCE AP-                                       PRODUCT  PROXIMATELY 50% CELL GROWTH RE-                                       No.      DUCTION μg/ml MEDIA                                                ______________________________________                                          1       10.0-25.0                                                             16       20.0-50.0                                                             25       12.5-25.0                                                             ______________________________________                                    

                  TABLE 4                                                          ______________________________________                                         INHIBITION OF CELL GROWTH: HUMAN OESOPHAGAL                                    CARCINOMA HOC                                                                           MINIMUM INHIBITORY CONCENTRA-                                                  TIONS (RANGE) WHICH PRODUCE AP-                                       PRODUCT  PROXIMATELY 50% CELL GROWTH RE-                                       No.      DUCTION μg/ml MEDIA                                                ______________________________________                                         1        25.0-50.0                                                             2         50.0-100.0                                                           3        25.0-50.0                                                             5        ≧25.0                                                          6        10.0-20.0                                                             23       20.0-50.0                                                             ______________________________________                                    

                  TABLE 5                                                          ______________________________________                                         INHIBITION OF CELL GROWTH: HUMAN CERVICAL                                      CANCER HeLa ATCC No. CCL2                                                               MINIMUM INHIBITORY CONCENTRA-                                                  TIONS (RANGE) WHICH PRODUCE AP-                                       PRODUCT  PROXIMATELY 50% CELL GROWTH RE-                                       No.      DUCTION μg/ml MEDIA                                                ______________________________________                                          1       20.0-40.0                                                              2       50.0-75.0                                                              3       40.0-60.0                                                              6        60.0-100.0                                                            8        60.0-100.0                                                            9        60.0-100.0                                                           10       25.0-50.0                                                             11       25.0-50.0                                                             13        50.0-100.0                                                           15       25.0-50.0                                                             16       25.0-50.0                                                             17       20.0-25.0                                                             18       25.0-50.0                                                             19       20.0-40.0                                                             20       25.0-50.0                                                             21       25.0-50.0                                                             23        60.0-100.0                                                           24       25.0-50.0                                                             25        5.0-10.0                                                             26       20.0-40.0                                                             29       50.0                                                                  30       50.0                                                                  31       25.0-50.0                                                             ______________________________________                                    

                  TABLE 6                                                          ______________________________________                                         INHIBITION OF CELL GROWTH: P27-HUMAN                                           MESOTHELIOMA                                                                            MINIMUM INHIBITORY CONCENTRA-                                                  TIONS (RANGE) WHICH PRODUCE AP-                                       PRODUCT  PROXIMATELY 50% CELL GROWTH RE-                                       No.      DUCTION μg/ml MEDIA                                                ______________________________________                                          1       10.0-25.0                                                              3       25.0-50.0                                                              8       40.0-60.0                                                             16       25.0-50.0                                                             17       20.0-50.0                                                             ______________________________________                                    

                  TABLE 7                                                          ______________________________________                                         INHIBITION OF CELL GROWTH: "LOOT'S" CELLS                                      HUMAN ADENOCARCINOMA                                                                    MINIMUM INHIBITORY CONCENTRA-                                                  TIONS (RANGE) WHICH PRODUCE AP-                                       PRODUCT  PROXIMATELY 50% CELL GROWTH RE-                                       No.      DUCTION μg/ml MEDIA                                                ______________________________________                                          1       12.5-25.0                                                             16       12.5-25.0                                                             23       25.0-50.0                                                             ______________________________________                                    

                  TABLE 8                                                          ______________________________________                                         INHIBITION OF CELL GROWTH: CHANG CONJUNCTIVAL                                  ATCC No. CCL20.2                                                                        MINIMUM INHIBITORY CONCENTRA-                                                  TIONS (RANGE) WHICH PRODUCE AP-                                       PRODUCT  PROXIMATELY 50% CELL GROWTH RE-                                       No.      DUCTION μg/ml MEDIA                                                ______________________________________                                          1       20.0-25.0                                                             23       25.0-75.0                                                             25        2.5-10.0                                                             26        2.5-10.0                                                             ______________________________________                                    

                  TABLE 9                                                          ______________________________________                                         ACUTE TOXICITY OF SOME PENT-4-EN-1-YNES                                        AND DERIVATIVES.                                                                        MINIMUM TOLERATED SINGLE DOSE                                         PRODUCT  mg/kg BODY WEIGHT                                                     No.      MICE        RATS        ROUTE                                         ______________________________________                                         P.sub.2   ≧500                                                                               ≧250 P.O.                                                    ≧200                                                                               ≧100 I.P.                                           2       ≧1000                                                                                500        P.O.                                           3         500        1500       P.O.                                          11       ≧1000            P.O.                                          16         500        500        P.O.                                          17         500        500        P.O.                                          19         250                   P.O.                                          23         750                   P.O.                                          ______________________________________                                          NOTE:                                                                          ≧ more than                                                             P.O. by oral route                                                             I.P. by intraperitoneal route                                             

We claim:
 1. A compound of the general formula: ##STR89## R¹ is a hydrogen or taken together with R² is a keto group, R² is a hydrogen or a hydroxyl group,R³ is a hydrogen or an acyl group, R⁴ is a hydrogen or a halogen, A and B are the same or different in that A represents phenyl, substituted phenyl or CH₂ OR group (in which R is H, lower alkyl, aralkyl or acyl) and B represents H, phenyl, substituted phenyl, furyl--(CH₂)_(n) COOH (n=0-5), or alkyl the aforesaid subject to the proviso that if:i. R¹ =R² =R³ =R⁴ =H then (a) A is not C₆ H₅ when B is H or CH₃ ii. R² =OH, R¹ =R³ =R⁴ =H and A=C₆ H₅ --, then B is not CH₃, C₆ H₅ -- or m-chlorophenyl, iii. R¹ and R² =0 and R³ =R⁴ =H, then(a) both A and B simultaneously are not phenyl-, furyl-, thienyl-, pyryidyl- or norbornyl groups, (b) A is not C₆ H₅ -- and B is not H.
 2. A compound as in claim 1 comprising a pent-4-en-1-yne compound of the formula ##STR90## in which A is chosen from the group including a substituted phenyl group and a --CH₂ OH group.
 3. A compound as in claim 1 comprising a pent-4-en-1-yne compound of the formula ##STR91## in which A is chosen from the group including a substituted phenyl group, --CH₂ --OH and H.
 4. A compound as in claim 1 including a pent-4-en-1-yne compound of the formula ##STR92## in which A is a chosen from the group including a substituted phenyl group and --CH₂ OH group.
 5. A compound including a pent-4-en-1-yne compound of the formula ##STR93## in which A is chosen from the group including a substituted phenyl group and --CH₂ -OH group.
 6. A compound as in claim 1 including a pent-4-en-1-yne compound of the formula ##STR94## in which R¹ and R² are chosen from H and OH or when taken together are =0.
 7. A compound as in claim 1 comprising a pent-4-en-1-yne compound of the formula ##STR95## in which R¹ and R² are chosen from H and --OH, or when taken together are =0; R¹ 1, R¹ 2, R¹ 3 and R¹ 4 are chosen from --OMe, OH and --O-Glycoside.
 8. A compound as in claim 1 comprising a pent-4-en-1-yne compound of the formula ##STR96## in which B is chosen from H, a phenyl group, a substituted phenyl group, an alkyl group, --(CH²)_(n) COOH (with n=0-5); R¹ 1 and R¹ 2 are chosen from H,OH, --OMe and --O-Glycoside.
 9. A compound as in claim 1 comprising a pent-4-en-1-yne compound of the formula ##STR97## in which B is chosen from H, a phenyl group, substituted phenyl group, an alkyl group and --(CH₂)_(n) COOH (n=0-5).
 10. A compound as in claim 1 comprising a pent-4-en-1-yne compound of the formula ##STR98## in which B is chosen from a substituted phenyl group, --(CH₂)_(n) COOH (n=0-5) and a furyl group.
 11. A compound as in claim 1 comprising a pent-4-en-1-yne compound of the formula ##STR99## in which B is chosen from a substituted phenyl group, an alkyl group, --(CH₂)_(n) COOH (n=0-5) and a furyl group.
 12. A compound as in claim 1 of the formula: ##STR100##
 13. A composition comprising 10 to 500 mg of a compound of the general formula: ##STR101## in which R¹ is a hydrogen or taken together with R² is a keto group,R² is a hydrogen or a hydroxyl group, R³ is a hydrogen or an acyl group, R⁴ is a hydrogen or a halogen, A and B are the same or different in that A represents an alkyl- or aryl group where all such alkyl-, aryl- and acyl groups may be variously substituted; and a pharmaceutically acceptable carrier.
 14. A composition comprising 10 to 500 mg of a compound of the formula ##STR102## and a pharmaceutically acceptable carrier. 